Obtain the plasmid-bearing bacterial cells1. Collect the three bacterial cultures that your group has been assigned. The bacteria have been transformed with plasmids containing either an S. cerevisiae MET gene, its S. pombe ortholog or bacterial LacZ. Each culture contains 0.6 mL Luria Bertani (LB) medium with 100 μg/mL ampicillin.
These instructions are designed for constructing two 12 SDS-PAGE gels with the BioRad Mini Protean system. The second gel will be used for western blotting.Assemble the gel casting apparatusAssemble the components that you will need for casting the gel: a tall glass plate with attached 1 mm spacers, a small glass plate, a green casting frame and a casting stand.
I'm a scientist who has significant experience in chemistry but am relatively new to molecular biology and biochemical techniques. I'm trying to make an isolated domain of a protein (166 residues,…
Apparemment, cela a conduit à des résultats ayant une signification clinique, comme nous pouvons le voir sur http://extremelongevity.net/2011/10/03/daily-fish-oil-consumption-may-reduce-lifespan/… Les chercheurs ont nourri un s…
I am a student and I'm currently using the IB biology Oxford textbook. A few weeks ago I had a test on biochemistry. I studied on my textbook and it stated the following: "Mitochondrion: (…) fat is
