Our lab uses old Gilson Pipetman pipettes. I've been using some for making mRNA by in vitro transcription and started running into RNAse issues. I'm trying to clean everything up, including the pip…
Μια εικόνα από αυτήν την ερώτηση (και αρχικά από το μοντέλο υπολογιστή του Karl Niklas) έχει προκαλέσει την ακόλουθη ερώτηση: Τα κλαδιά σε αυτό το δέντρο διαιρούνται με αυξανόμενη συχνότητα καθώς προχωράμε από το
Ποιες είναι οι διαφορές μεταξύ των παρακάτω ικανών κυττάρων DH5 alpha, Nove blue και BL21; Γιατί μερικά από αυτά είναι για κλωνοποίηση και άλλα για έκφραση πρωτεΐνης; Οποιεσδήποτε αναφορές όπου θα μπορούσα να βρω…
Learning Objectivesstate the type of oxidizing reagent used and the products forms on oxidation reactions catalyzed by dehydrogenases, monoxygenases (hydroxylases), dioxygenases, and oxidases;draw the reactive end of NAD+ and mechanisms showing it& 39;s reactions with substrates in enzyme-catalyzed two electron oxidation reactions;explain differences in chemical reactivity of NAD+ and FAD in one and two electrons oxidations and with dioxygen;describe the stereochemistry of the alcohol dehydrogenase-catalyzed oxidation of prochiral ethanol by NAD+;explain why FAD/FADH2 are often tightly bound to dehydrogenases in contrast to NAD+/NADH where are freely diffusable substrates;given standard reduction potentials, determine the ΔGo& 39; for given redox reactions;explain why different FAD and other flavin containing dehydrogenases have varying standard reduction potentials for the flavin but NAD+ dependent dehydrogenase have only one;describe the role of heme in mono- and dioxygenases in activating dioxygen and minimizing side reactions of ROSs;describe the biological role of cytochrome P450s;define and give examples of oxidases;compare the contrast the role of the heme in carrying hemoglobin and myoglobin, monoxygenases, and in oxidases.
What is the equation for cellular respiration in carnivores as they don’t consume carbohydrates to break down into glucose in the following manner: Glucose + oxygen -> water + CO2 +energy. Do th…